Vascular changes and circulatory stagnation are commonly considered to be the main mechanism of biological tissue injury during low temperature exposure. Properties of the pancreas parenchyma response during the phenomenon of the " lunar eclipse" provide important insights into the mechanisms of damage and the formation of cryogenic lesion immediately after thawing in cryosurgery. The " lunar eclipse" phenomenon contributes to a fundamental understanding of the mechanisms of biological tissue damage during low temperature exposure in cryoscience and cryomedicine. This snow-white cryogenic lesion dissolved in the same manner in all animal tissues. Immediately after freezing, during the thawing process, the snow-white pancreas parenchyma, frozen hard to an ice block and resembling a full moon with a sharp demarcation line, gradually assumed a ruby-red shade and a hemispherical shape as it grew in size depend on reconstruction vascular circulation from the periphery to the center. Each cryolesion was observed for one hour after thawing intraoperatively. The freeze-thaw cycle was monitored by intraoperative ultrasound before, during and after cryosurgery. For cryosurgical exposure a temperature range of -40 degrees C, -80 degrees C, -120 degrees C and -180 degrees C was selected in contact with pancreas parenchyma. A disc cryogenic probe was placed on the pancreas after the laparotomy. In this experimental study 76 animals (mongrel dogs) were investigated. Similar phenomena author has observed in nature, namely the total lunar eclipse and total solar eclipse. The present investigations describe a unique phenomenon, namely the phenomenon of the " lunar eclipse", which has been observed and discovered by the author in living substance during the freeze-thawing processes in vivo using temperatures of various intensities and its cryosurgical response in animal experiment. Living matter: the " lunar eclipse" phenomena.
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